Lyophilized Exosomes CD63-EGFP from HEK293 cells
These purified exosomes (small EVs) were isolated from human kidney cells (HEK293 cell line) by a combination of tangential flow filtration (TFF) and size exclusion chromatography (SEC). They express the fluorescent protein EGFP as fusion protein with the tetraspanin CD63. The fluorophore is bright and highly stable and can therefore be used for in vitro tracking studies or for assay calibration and verification for various techniques, like F-NTA, Flow Cytometry, ELISA or Western Blot.
They are delivered in lyophilized form for long term stability (up to 12 month).
Product details
Please refer to our product details for specifications, protocols, and the Safety Data Sheet for comprehensive insights into performance, usage and safety.
Product specifications
Size: 10µg (#700378) / 50µg (#700377)
Origin: human embryonic kidney (HEK293 cells)
GPF Excitation / Emission: 488nm / 507nm
Isolation:
Storage conditions: 4°C
Protocol
For detailed product specifications and application guidelines, refer to our Protocol.
Safety Data Sheet
For safety instructions and handling recommendations, consult the Safety Data Sheet: Link.
Support
If you have any questions about this product or its optimal use in your research, our support team is happy to assist you. Contact us at support@particle-metrix.com – we’re here to help!
Applications
Lyophilized Exosomes can be used as positive controls as well as for assay calibration and verification for various techniques. The quantities provided below are examples and may vary depending on experimental conditions and specific assay requirements.
| Technique | Recommended Quantity (example values) |
|---|---|
| Fluorescence NTA | 5µg |
| Flow Cytometry | 5µg |
| ELISA | 20 - 40µg per well |
| Western Blot | 20 - 40µg per line |
