Concentration Scanning Technology
Particle concentration is a critical parameter for understanding sample composition, consistency and performance. With Particle Metrix’s new Concentration Scanning Technology, integrated into all ZetaView® Evolution systems and powered by the intuitive ZetaSphere software, researchers can now achieve calibration-free, highly reproducible particle concentration measurements – across a wide range of particle sizes, materials and fluorescent labels.
What is Particle Concentration?
Particle concentration refers to the number of particles per milliliter in a liquid suspension. It is a vital metric in numerous applications – from exosome research and virus analysis to drug delivery and materials science. Precise concentration data are crucial for a sample qualification since they can ensure reproducibility, product safety and accurate dosing.
Why is Particle Concentration important?
Knowing the exact number of particles in a sample is essential to:
- Standardize and compare experimental results
- Ensure consistency in pharmaceutical formulations
- Optimize protocols in environmental and life science applications
- Maintain high quality in nanomaterial production
New Concentration Scanning Technology
Unlike traditional NTA methods that rely on fixed positions and calibration with reference materials, our Concentration Scanning Technology performs a continuous volumetric scan of the entire measurement volume. This unique approach offers:
- Calibration-Free Measurement: No reference material required, even for particles with unknown size or fluorescence.
- High Reproducibility: Consistent results widely independent of camera or laser settings.
- Wide Applicability: Accurate quantification of scatter and fluorescent particles, from quantum dots, over extracellular vesicles, viruses and nanobubbles to nanometals.
- Improved Cross-Device Comparability: Reliable data across different ZetaView® systems.
- Elevated Statistics: in a measurement volume of 220nl up to 20.000 particles can be measured within seconds for reliable and statistically robust results.
How Does It Work?
Using Nanoparticle Tracking Analysis (NTA) and the new scan-based approach, ZetaView® Evolution systems:
- Continuously scan the entire measurement volume.
- Detect thousands of particles in just seconds - across scatter and fluorescence channels.
- Widely eliminate sensitivity to camera gain or laser intensity variations.
- Output high-confidence concentration values without time-consuming calibrations.
Key Advantages of ZetaView® Evolution with Concentration Scanning:
Multi-Parameter Analysis: measure size, concentration, fluorescence, zeta potential and colocalization of one sample in up to 11 channels with just one click
Exceptional Sensitivity: Detect particles as small as 10nm in scatter or with down to 20 AlexaFluor488 molecules in fluorescence.
Fast Setup, Easy Operation: Streamlined workflows and minimal user intervention.
Robust for Real Samples: Accurate results even with complex or biologically variable samples like EVs.
Applications of Concentration Scanning Technology
Concentration analysis with ZetaView® Evolution supports:
Pharmaceuticals: For consistent nanoparticle-based drug delivery and quality assurance.
Biotech & Life Sciences: Reliable EV and virus quantification in scatter and fluorescence mode.
Environmental Studies: Measurement of nanoparticle contamination
Materials Science: Characterization of synthetic and functionalized nanoparticles.
Nanobubbles: Reproducible quantification of ultrafine or nanobubbles for use in water treatment, beverage production and other applications.
Let’s talk about your Particle Concentration Scanning needs
Discover how Concentration Scanning Technology can transform your nanoparticle research.
Contact our experts today for a personalized consultation or system demonstration.
Frequently asked questions about Particle Concentration
What types of samples can be analyzed for particle concentration?
Size distribution can be measured for all nanoparticles (e.g. extracellular vesicles, nano bubbles, nano metals) within a size range of 10* – 1000nm and a concentration range of 105 – 109 particles/ml.
(*sample and laser dependent)
What are the advantages of using Particle Metrix’s technology for Particle Concentration analysis?
Our scanning technology allows the detection of a large sample volume in less than one minute, analyzing thousands of particles in one run. Therefore, our ZetaView delivers statistically highly relevant data.
How do I ensure consistent and accurate Particle Concentration measurements?
The keys to ensuring reliable particle size measurements is a consistent sample preparation as well as the use of a suitable operation protocol as delivered during the instrument training. Additionally, integrated algorithms control consistent measurement conditions through the whole sample volume and indicate outliers.
Can the ZetaView® system measure fluorescently labeled particles?
Yes, the system supports fluorescence measurements, allowing detailed analysis of labeled subpopulations within your sample. Depending on the chosen model (MONO, TWIN, QUATT) and the number of implemented fluorescence filters, the ZetaView features the analysis of up to 10 different fluorescent channels.
